祝诚
- 教师名称:祝诚
- 教师拼音名称:Zhu Cheng
- 性别:男
- 职务:英才副教授,特聘研究员
- 学科:Biochemistry and Biophysics
其他联系方式
- 通讯/办公地址:
- 邮箱:
基本信息
研究方向
获奖情况
论文成果
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蛋白质稳态与动态结构的理性设计
祝诚研究组综合运用生物化学、生物物理等实验技术,结合计算机辅助设计、分子动力学模拟等计算方法,理性设计与改造蛋白质分子的结构与功能,为更精确的抗体设计、酶的改性、正交化生物体系的构建提供新方法和新元件。
1、蛋白质稳态结构的设计,赋予多种蛋白质骨架全新的功能
人工智能驱动的双功能分子序列设计 (eLife 2024)
连续结构片段嫁接(Nature Communications 2019, Methods in Molecular Biology 2017)
分离活性位点嫁接(Protein Science 2016, Protein & Cell 2011)
2、蛋白质动态构象的设计,开发非稳态结构捕捉与调控方法
蛋白质解折叠中间体(PNAS 2018, Structure 2016)
蛋白质折叠过渡态(Biophysical J 2013)膜蛋白激活态(Proteins 2019)
3、动力学模拟解析复杂生命分子的构造规律
合作发表:蛋白质-核酸(Immunity 2019, PAIN 2023)蛋白质-气体 (Nature Cell Biology 2022) 蛋白质-高分子聚合物(Nature Communications 2022)蛋白质-小分子(Microbiology Spectrum 2021)小分子-膜 (Advanced Science 2023) 多肽-膜 (Frontiers in Immunology 2022) 蛋白质-无机纳米粒子 (Frontiers in Molecular Biosciences 2021)
承担科研项目
国家自然科学基金 面上项目 理性设计别构调控的蛋白质活性开关 (主持)
国家自然科学基金 青年项目 理性设计表位嫁接的HIV-1 Env蛋白质抗原 (主持)
国家自然科学基金 原创探索计划项目 未来生物技术(参与)
科技部 国家重点研发计划 正交化蛋白质元件的人工设计与构建 (参与)
天津大学 自主创新基金 冠状病毒复制启动蛋白的动力学与进化模式研究 (主持)
研究应用
HIV-1新型抗原:Science Daily 渐冻症与SOD1蛋白质聚集:F1000 Recommendation 多肽抗菌/抗病毒机制模拟:Frontiers 磷酸酶如何感知CO2: Intrinsically disordered CO2 sensors 膜融合脂质体如何递送物质入细胞: Advanced Science封面
专利
末端脱氧核苷酸转移酶突变体及其应用、miRNA 检测方法
抗微生物肽Perceptide-TJ-2及其在制备广谱抗病毒药物中的用途(ZL 2023 1 0894433.5)
一种抗菌肽及其在制备抗菌药物或抗病毒药物中的应用
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- Yuqiong Xia, Keyun Wu, Chang Liu, Xuejuan Zhao, Jun Wang, Jianxia Cao, Zhaoxu Chen, Minchao Fang, Jie Yu, Cheng Zhu, Xianghan Zhang*, Zhongliang Wang* “Filamentous-Actin-Mimicking Nanoplatform for Enhanced Cytosolic Protein Delivery” Advanced Science, 2023, e2305600, doi: 10.1002/advs.202305600
- Rongrong Liu, Yunhua Lv, Wenjie Sun, Min Li, Ningning Ge, Cheng Zhu, Yaxin Ding, Ziyu Liu, Ruixue Ma, Yuxiao Huang, Shiyuan Hou, Qikang Ying, Tianle Gu, Fang Wang, Lingling Nie, Youchun Wang, Weijin Huang*, Jiayi Shu*, Xingan Wu* “Investigation of a subunit protein vaccine for HFRS based on a consensus sequence between envelope glycoproteins of HTNV and SEOV” Virus Research, 2023, 334:199149
- Jun Kang, Mengqian Huang, Jinyu Li, Keke Zhang, Cheng Zhu, Sihua Liu, Zhenwei Zhou, Tao Wang*, Zhiyun Wang* "Enterovirus D68 VP3 Targets the Interferon Regulatory Factor 7 To Inhibit Type I Interferon Response" Microbiology Spectrum, 2023, 11(3): e0413822
- Xun Sun, Jing Yu, Cheng Zhu, Xinreng Mo, Qiangqiang Sun, Dandan Yang, Chang Su*, Yang Lu* "Recognition of galactose by a scaffold protein recruits a transcriptional activator for the GAL regulon induction in Candida albicans" eLife, 2023, 12: e84155
- Yanyan Wu, Qian Wang, Pingan Zhang, Cheng Zhu, Guangyin Xu* "miR-1306-3p directly activates P2X3 receptors in primary sensory neurons to induce visceral pain in rats" PAIN, 2023, 164(7):1555-1565
- Zhuozhi Chen, Rongdi Duan, Yunjie Xiao, Yi Wei, Hanxiao Zhang, Xinzhao Sun, Shen Wang, Yingying Cheng, Xue Wang, Shanwei Tong, Yunxiao Yao, Cheng Zhu, Haitao Yang, Yanyan Wang*, Zefang Wang* "Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin" Nature Communications, 2022, 13(1):7138
- Mao Zhang, Cheng Zhu, Yuanyuan Duan, Tongbao Liu, Haoping Liu, Chang Su*, Yang Lu* "The intrinsically disordered region from PP2C phosphatases functions as a conserved CO2 sensor" Nature Cell Biology, 2022, 24(7): 1029–1037
- Rongrong Liu, Ziyu Liu, Haoran Peng, Yunhua Lv, Yunan Feng, Junjun Kang, Naining Lu, Ruixue Ma, Shiyuan Hou, Wenjie Sun, Qikang Ying, Fang Wang, Qikang Gao, Ping Zhao, Cheng Zhu*, Yixing Wang*, Xingan Wu* "Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses" Frontiers in immunology 2022, 13: 851642
- Yaxin Wang, Binghong Xu, Sen Ma, Hao Wang, Luqing Shang, Cheng Zhu*, Sheng Ye*, "Discovery of SARS-CoV-2 3CLPro Peptidomimetic Inhibitors through the Catalytic Dyad Histidine-Specific Protein-Ligand Interactions", Int J Mol Sci. 2022, 23(4):2392
- Binghong Xu, Meijun Liu, Sen Ma, Yuying Ma, Si Liu, Luqing Shang, Cheng Zhu*, Sheng Ye*, Yaxin Wang*, "4-Iminooxazolidin-2-One as a Bioisostere of Cyanohydrin Suppresses EV71 Proliferation by Targeting 3C pro" Microbiology Spectrum 2021: e0102521
- C. Zhu, X. Zhou, Z. Liu, H. Chen, H. Wu, X. Yang, X. Zhu, J. Ma*, H. Dong* “The morphology of hydroxyapatite nanoparticles regulates cargo recognition in clathrin-mediated endocytosis” Frontiers in Molecular Bioscience 2021 8:627015
- J.Y Cheng, S. Gao, C. Zhu, S.H. Liu, J.Y. Li, J. Kang, Z. Wang, T. Wang* "Typical Stress Granule Proteins Interact with the 3' Untranslated Region of Enterovirus D68 To Inhibit Viral Replication" Journal of Virology 2020, 94, e02041-19
- C. Zhu, E. Dukhovlinova, O. Council, L.H. Ping, E.M. Fasion, G.W. Yin, E.L. Potter, L. Kincer, S.L. Campbell, S.R. Benhabbour, H.M. Ke, R. Swanstrom, N.V. Dokholyan, " Rationally Designed Carbohydrate-Occluded Epitopes Elicit HIV-1 Env-Specific Antibodies" Nature Communications 2019, 10, 948
- X. Li, M. Deng, A. Petrucelli, C. Zhu, J.Y. Mo, L. Zhang, J.W. Tam, P. Ariel, B.Y. Zhao, S. Zhang, H.M. Ke, N.V. Dokholyan, J.A. Duncan, J.P.Y. Ting, "Viral DNA Binding to NLRC3, an Inhibitory Nucleic Acid Sensor, Unleashes STING, a Cyclic Dinucleotide Receptor that Activates Type I Interferon" Immunity 2019, 50, 591-599
- C. Zhu, Q. Han, A. Samoshkin, M. Convertino, A. Linton, E.M. Fasion, R. Ji, L. Diatchenko and N.V. Dokholyan*, "Stabilization of mu-opioid receptor facilitates its cellular translocation and signaling", Proteins 2019, 87, 878-884.Proteins: Structu...,87 (10):878-884
- C. Zhu, M.V. Beck, J.D. Griffith, M. Deshmukh, N.V. Dokholyan, "Large SOD1 aggregates, unlike trimeric SOD1, do not impact cell viability in a model of amyotrophic lateral sclerosis" Proc. Natl. Acad. Sci. USA 2018, 115, 4661-4665
- F.M. James, C. Zhu, E. Proctor, Y. Z. Tao, W.J. Cui, H.M. Ke, N.V. Dokholyan, "A Phosphomimetic Mutation Stabilizes Cu, Zn Superoxide Dismutase and Rescues Cell Viability in the Context of an ALS-associated Mutation" Structure 2016, 24, 1898-1906
- C. Zhu, C.S. Zhang, T. Zhang, X.L. Zhang, L.H. Lai, "Rational Design of TNFα Binding Proteins Based on the De Novo Designed Protein DS119" Protein Sci. 2016, 25, 2066-2075
- C. Zhu, Z.W. Dai, H.H. Liang, T. Zhang, F. Gai, L.H. Lai, "Slow and bimolecular folding of a de novo designed monomeric protein DS119" Biophys. J. 2013, 105, 2141-2148
- C. Zhu, C.S. Zhang, H.H. Liang, L.H. Lai, "Engineering a zinc binding site into the de novo designed protein DS119 with a beta-alpha-beta structure" Protein & Cell 2011, 2, 1006-1013
教育经历
- 2007.9-2013.7 北京大学 物理化学 博士 导师 来鲁华教授
- 2003.9-2007.6 南京大学 化学 学士 导师 杜学忠教授
工作经历
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2019.9 - 至今
|生命科学学院|天津大学|英才副教授
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2018.6 -2019.9
|药学系|美国宾州州立大学|导师 Prof. Nikolay V. Dokholyan|博士后
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2013.10 -2018.6
|生物化学与生物物理系|美国北卡罗来纳大学教堂山分校|导师 Prof. Nikolay V. Dokholyan|博士后