School of Chemical Engineering and Technology
Professor
Pharmaceutical Engineering
mindong@tju.edu.cn
Peiyang Campus 50-A509
Dr. Dong is a professor in the Department of Pharmaceutical Engineering, School of Synthetic Biology and Biomanufacturing, Tianjin University, and a Principle Investigator of the State Key Laboratory of Synthetic Biology. He joined Tianjin University in 2019. His research group focuses on the fields of Chemical and Synthetic Biology, with research interests ranging from natural product biosynthesis, novel enzymatic reactions, biocatalysts development, and mechanistic studies on metalloproteins to mechanism-based inhibitor design and synthesis for drug discovery. He has published multiple papers in Science, J. Am. Chem. Soc., Angew.Chem.Int.Ed., ACS Catalysis, et al.
- Doctoral degree| Peking University| 化学生物学
- Master’s Degree| Tianjin University| 有机化学
- Bachelor’s Degree| Tianjin University| 制药工程
- 1) Metalloproteins discovery, mechanistic study and engineer
2) Natural products biosynthesis
3) Chemical and Synthetic biology of SAM related enzymes, including methyltransferases, radical SAM enzymes, and ACP transferases.
4) Mechanism based inhibitor design and synthesis for drug dis
- Papers
- [1] Zhen. X.; Chen, F.; Dong, M.*.Biosynthesis of Methylazetidinecarboxylic Acid with Opposite Stereoconfigurations in Vioprolides and Bonnevillamides. ACS Catalysis. 2025, 15, 18556-18562
- [2] Zhao H.; Yu, N.; Wang, S.; Dong, M.*.Cyclopropylmethyl S-adenosyl-L-methionine: an enzymatic cyclopropylmethyl donor. Chem. Sci., 2025,16, 45, 21483-21488
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- [3] Wei, Z.; Yu, N.; Xu, T.; Liu, Z.; Li, B.; Dong, M.* Reductive Photoenzymatic O-Demethylation of Aryl Methyl Ethers and Lignin Monoaromatics Produces Methane. ACS Catalysis. 2025, 15, 16, 13712–13719
- [4] Wu, X.; Dong, M.* Photoaffinity SAM analogues for the identification of SAM-binding proteins. Chem. Sci. 2025,16, 12043-12050
- [5] Kong, L.; Zhang, J.; Wang, H.; Wei, Z.; Wang, W.; Hu, J.*; Dong, M.* B12‐Dependent Radical SAM Enzymes Catalyze C‐Fluoromethylation via a CH2F‐Cobalamin Intermediate. Angew. Chem. Int. Ed. 2025, e202419815
- [6] Yao, Y.; He, J.; Chen, F.; Dong, M.*. Arsinothricin Biosynthesis Involving a Radical SAM Enzyme for Noncanonical SAM Cleavage and C-As Bond Formation. J. Am. Chem. Soc. 2024, 146, 21214-21219
- [7] Jiang, Y.; Yao, M.; Feng, J.; Niu, H.; Qiao, B.; Li, B.; Wang, B.*; Xiao, W.*; Dong, M.*, Yuan, Y. Molecular Insights into Converting Hydroxide Adenosyltransferase into Halogenase. J Agric Food Chem 2024, 72, 12685-12695
- [8] Yu, N.; Zhao H.; Wang, W.; Dong, M.*. Enzymatic fluoroethylation by a fluoroethyl selenium analogue of S-adenosylmethionine. ACS Catalysis. 2024, 14, 6211–6216
- [9] Jiang, Y.; Yao, M.; Niu, H.; Wang, W.; He, J.; Qiao, B.; Li, B.; Dong, M.*, Xiao, W.*; Yuan, Y. Enzyme Engineering Renders Chlorinase the Activity of Fluorinase. J Agric Food Chem 2024, 72, 1203-1212.
- [10] Wang, W.; Zhao H.; Yu, N.; Chen, F.; Dong, M.*. Stable SAdenosylmethionine Analogue for Enzymatic Fluoromethylation. ACS Catalysis. 2023, 13, 13729-13734
- [11] Huang, R.; Zhi, N.; Yu, L.; Li, Y.; Wu, X.; He, J.; Zhu, H.; Qiao, J.; Liu, X.; Tian, C.; Wang, J *; Dong, M.* Genetically encoded photosensitizer protein reduces iron-sulfur clusters of radical SAM enzymes. ACS Catalysis, 2023, 13, 1240-1245.
- [12] Zhi, N.; Zhu, H.; Qiao, J.; Dong, M.* Recent progress in radical SAM enzymes: New reactions and mechanisms. Chin. Sci. Bull 2022, 67, 1806-1820
- [13] Li, Y.; Yao, Y.; Yu, L.; Tian, C.; Dong, M.* Mechanistic investigation of B12-independent glycerol dehydratase and its activating enzyme GD-AE. Chem. Commun., 2022, 58, 2738-2741
- [14] Zhou, S.; Chen, X.; Ni, X*.; Liu, Y.; Zhang, H.; Dong, M.*; Xia, H*. Pyridoxal-5´-phosphate-dependent enzyme GenB3 Catalyzes C-3´,4´-dideoxygenation in gentamicin biosynthesis. Microb. Cell. Fact. 2021, 20:65, 1-12.
- [15] Fenwick, M. K.; Su, Dan.; Dong, M.; Lin, H *; Ealick, S. E.* Structural Basis of the Substrate Selectivity of Viperin. Biochemistry 2020, 59, 652–662.
- [16] Dong M, Dando EE, Kotliar I, Su X, Dzikovski B, Freed JH, Lin H. The asymmetric function of Dph1-Dph2 heterodimer in diphthamide biosynthesis. J. Biol. Inorg. Chem. 2019, 24, 777-782.
- [17] Fenwick, M. K.; Dong, M.; Lin, H.; Ealick, S. E.*. The Crystal Structure of Dph2 in Complex with Elongation Factor 2 Reveals the Structural Basis for the First Step of Diphthamide Biosynthesis. Biochemistry 2019, 58, 4343–4351.
- [18] Dong, M.; Kathiresan, V.; Fenwick, M. K.; Torelli, A. T.; Zhang, Y.; Caranto, J. D; Dzikovski, B.; Sharma, A.; Lancaster, K. M.; Freed, J. H.; Ealick, S. E.; Hoffman, B. M.; Lin, H. Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science 2018. 359, 1247-1250.
- [19] Dong, M.; Horitani, M.; Dzikovski, B.; Freed, J. H.; Ealick, S. E.; Hoffman, B. M.; Lin, H. Substrate-Dependent Cleavage Site Selection by Unconventional Radical S Adenosylmethionine Enzymes in Diphthamide Biosynthesis. J. Am. Chem. Soc. 2017. 139, 5680-5683
- [20] Dong, M#.; Horitani, M#.; Dzikovski, B.; Pandelia, M. E.; Krebs, C.; Freed, J. H.; Hoffman, B.; Lin, H. Organometallic complex formed by an unconventional radical SAM enzyme. J. Am. Chem. Soc. 2016. 138, 9755-9758.




