Xuan Weimin
School
School of Life Sciences School of Life Sciences
Professional Title
Professor
Administrative Appointments
无
Discipline
Chemical Biology and Synthetic Biology
Contact Information
weimin_xuan@tju.edu.cn
卫津路92号 第14教学楼307
300072
Education Background
- Doctoral degree| 美国新墨西哥大学| 化学| 2013
- Bachelor’s Degree| 天津大学| 药学| 2007
Research Interests
- Chemical Biology/Synthetic Biology based on Genetic Code Expansion
- Evolution and Design of Functionally innovative Proteins
- Protein/Peptide-based Drug Discovery via Phage Display
Positions & Employments
-
2022.5-Now
天津大学生命科学学院 | 教授 -
2017.12-2021.11
南开大学 | 特聘研究员 -
2013.8-2017.9
美国斯克里普斯研究所 | 博士后
Academic Achievements
- Papers
- [1] Qifan Wu,# Xiaochen Yang,# Ying Wang, Hongqiang Qin, Xun-Cheng Su, Weimin Xuan,* Site-Selectively Accelerating the Generation of β-Linked Residue Isoaspartate in Proteins, Angew. Chem. Int. Ed. 2025, DOI: 10.1002/anie.202500983.
- [2] Ying Wang, Lin Sun, Weimin Xuan,* Genetically Encoded Fluorescent and Bioluminescent Probes for HDAC8, ChemBioChem 2025, e202500096.
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- [3] Lei Zhao, Zhifen Huang, Haonan Meng, Qianzhi Liang, Xun-Cheng Su, Weimin Xuan,* Site-Specific Protein Modification via Reductive Amination of Genetically Encoded Aldehyde. Bioconjugate Chem. 2025, acs.bioconjchem.4c00538.
- [4] Junhong Qin, Xinhe Huang, Shengsong Gou, Sitao Zhang, Yujie Gou, Qian Zhang, Hongyu Chen, Lin Sun, Miaomiao Chen, Dan Liu, Cheng Han, Min Tang, Zihao Feng, Shenghui Niu, Lin Zhao, Yingfeng Tu, Zexian Liu, Weimin Xuan, Lunzhi Dai, Da Jia,* Yu Xue,* Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation, Nat. Metab. 2024, https://doi.org/10.1038/s42255-024-01093-w
- [5] Xiaochen Yang, Xun-Cheng Su, Weimin Xuan,* Genetically Encoded Photocaged Proteinogenic and Non-Proteinogenic Amino Acids (invited review), ChemBioChem, 2024, 25(17):e202400393.
- [6] Weimin Xuan* and Jun-An Ma,* Pinpointing Acidic Residues in Proteins (invited concept), ChemMedChem, 2024, e202300623. doi: 10.1002/cmdc.202300623.
- [7] Yongan Wang,# Yanli Ji,# Lin Sun, Zhifen Huang, Sheng Ye,* Weimin Xuan,* A Sirtuin-Dependent T7 RNA Polymerase Variant, ACS Synth. Biol. 2024, 13, 1, 54–60, DOI: 10.1021/acssynbio.3c00607
- [8] Xiaochen Yang, Lei Zhao, Ying Wang, Yanli Ji, Xun-Cheng Su, Jun-An Ma, Weimin Xuan*, Constructing Photoactivatable Protein with Genetically Encoded Photocaged Glutamic Acid. Angew. Chem. Int. Ed. 2023, DOI:10.1002/anie.202308472.
- [9] W. Xuan and X. Yang., Chapter “Semi-synthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion” in book “Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols”. Methods Mol Biol. 2023, 2676:147-156.
- [10] Xin Chu, Bo Li, Hao-Yang Liu, Xiaowei Sun, Xiaochen Yang, Gang He, Chuanzheng zhou, Weimin Xuan, Shu-Lin Liu*, Gong Chen*, Bioconjugation via Hetero-Selective Clamping of Two Different Amines with ortho-Phthalaldehyde. Angew. Chem. Int. Ed. 2022, 61(31): e202205522. anie.202212199
- [11] Qifan Wu, Suwei Dong*, Weimin Xuan*, N-Glycan Engineering: Constructing the N-GlcNAc Stump (invited concept). ChemBioChem, 2023 Jan 3;24(1):e202200388.
- [12] Conghui Ren,# Qifan Wu,# Ruotong Xiao, Yanli Ji, Xiaochen Yang, Hongqiang Qin, Jun-An Ma, Weimin Xuan*, Expanding the scope of genetically encoded lysine post-translational modifications with lactylation, β-hydroxybutyrylation and lipoylation. ChemBioChem, 2022 Sep 16;23(18):e202200302.
- [13] Yanli Ji, Lin Sun, Yao Chen, Hongqiang Qin*, Weimin Xuan*, Sirtuin-Derived Covalent binder for the Selective Recognition of Protein Crotonylation. Angew. Chem. Int. Ed. 2022, 61(31): e202205522.
- [14] Qifan Wu,# Weidong Dong,# Hui Miao, Qian Wang, Suwei Dong,* Weimin Xuan*, Site-Specific Protein Modification with Reducing Carbohydrates, Angew. Chem. Int. Ed. 2022, 61(19): e202116545.
- [15] Ruotong Xiao, Lei Zhao, Hongpeng Ma, Qiaoli Liu, Hongqiang Qin, Xiaozhou Luo, Weimin Xuan*, Toward an Orthogonal Protein Lysine Acylation and Deacylation System, ChemBioChem, 2022, 23(4): e202100551.
- [16] Xiaochen Yang, Hui Miao, Ruotong Xiao, Luyao Wang, Yan Zhao, Qifan Wu, Yanli Ji, Juanjuan Du, Hongqiang Qin, Weimin Xuan*, Diverse Protein Manipulations with Genetically Encoded Glutamic Acid Benzyl Ester, Chem. Sci. 2021, 12(28), 9778 – 9785.
- [17] Wenbing Cao, Xuewen Qin, Yong Wang, Zhen Dai, Xianyin Dai, Haoyu Wang, Weimin Xuan, Yingming Zhang, Yu Liu, Tao Liu, A General Supramolecular Approach to Regulate Protein Functions by Cucurbit[7]uril and Unnatural Amino Acid Recognition, Angew. Chem. Int. Ed. 2021, 60(20):11196-11200.
- [18] Yanli Ji, Conghui Ren, Hui Miao, Zhili Pang, Ruotong Xiao, Xiaochen Yang and Weimin Xuan*, Genetically Encoding ε-N-Benzoyllysine in Proteins, Chem. Commun. 2021, 57(14), 1798 - 1801.
- [19] Hui Miao, Chenguang Yu, Anzhi Yao, Weimin Xuan*, Rational design of a function-based selection method for genetically encoding acylated lysine derivatives, Org. Biomol. Chem., 2019, 17(25), 6127 – 6130.
- [20] Xuan, W.;§ Collins, D.;§ Koh M.; Shao, S.; Yao, A.; Xiao, H.; Garner, PP.; Schultz, PG. Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins. ACS Chem. Biol. 2018, 13 (3), pp 578–581.
- [21] Xuan, W.;§ Yao, A.;§ Schultz PG. Genetically encoded fluorescent probe for detecting sirtuins in living cells. J. Am. Chem. Soc., 2017, 139 (36), pp 12350–12353.
- [22] Xuan, W.; Schultz, PG. A Strategy for Creating Organisms Dependent on Noncanonical Amino Acids. Angew. Chem. Int. Ed. 2017, 56(31), 9170-9173.
- [23] Xuan, W.; Shao, S.; Schultz, PG. Protein Crosslinking by Genetically Encoded Noncanonical Amino Acids with Aryl Carbamate Side Chains. Angew. Chem. Int. Ed. (hot paper) 2017, 56(18), 5096 –5100.
- [24] Xuan, W.; Li, J.; Luo, X.; Schultz, PG. Genetic Incorporation of a Reactive Isothiocyanate Group into Proteins. Angew. Chem. Int. Ed. 2016, 55(34), 10065-10068.
- [25] Xuan, W.; § Pan, R. § (equal contribution); Wei Y.; Cao, Y.; Li, H.; Liang, FS.; Liu, K.; Wang, W. Reaction-based “off-on” Fluorescent Probe Enabling Detection of Endogenous Labile Fe2+ and Imaging of Zn2+- induced Fe2+ Flux in Living Cells and Elevated Fe2+ in Ischemic Stroke. Bioconjugate Chem., 2016, 27, 302−308.
- [26] Xiao, H.;§ Xuan, W.§ (equal contribution); Shao, S.; Liu, T.; Schultz, P. G. Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones. ACS Chem. Biol. 2015, 10(7), 1599−1603.
- [27] Xuan, W.; Cao, Y; Zhou, J.; Wang, W. A FRET-based ratiometric fluorescent and colorimetric probe for the facile detection of organophosphonate nerve agent mimic DCP. Chem. Commun., 2013, 49, 10474—10476.
- [28] Xuan, W.; Pan, R.; Cao, Y.; Liu, K.; Wang, W. A fluorescent probe capable of detecting H2S at submicromolar concentrations in cells. Chem. Commun., 2012, 48, 10669-10671.
- [29] Xuan, W.; Chen, C.; Cao. Y.; He, W.; Jiang, W.; Liu, K.; Wang, W. Rational design of a ratiometric fluorescent probe with a large emission shift for the facile detection of Hg2+. Chem. Commun., 2012, 48, 7292–7294.
- [30] Xuan, W.; Sheng, C.; Cao, Y.; He, W.; Wang, W. Fluorescent Probes for the Detection of Hydrogen Sulfide in Biological Systems (highlights). Angew. Chem. Int. Ed. 2012, 51, 2282 – 2284.
- [31] Jack C Li, Fariborz Nastertorabi, Weimin Xuan, Gye Won Han, Raymond C Stevens, Peter G Schultz. A Single Reactive Noncanonical Amino Acid Is Able to Dramatically Stabilize Protein Structure ACS Chem. Biol. 2019, 14(6), 1150-1153.
- [32] Luo, X.; Fu, G.; Wang, R.; Zhu, X.; Zambaldo, C.; Liu, R.; Liu, T.; Lyu, X.; Du, J.; Xuan, W.; Yao, A.; Reed SA.; Kang, M.; Zhang Y.; Guo, H.; Huang, C.; Yang, PY.; Wilson, IA.; Schultz, PG.; Wang, F. Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria. Nat. Chem. Biol. 2017, 13, 845-849.
- [33] Zeng, G.; Li, H.; Wei, Y.; Xuan, W.; Zhang, R.; Breden, LE; Wang, W.; Liang, FS. Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity. ACS Synthetic Biology. 2017, 6 (6), pp 921–927.
- [34] Maston, L; Jones, D.; Giermakowska, W; Howard, TA; Cannon, JL.; Wang, W.; Wei, Y.; Xuan, W.; Resta, TC.; Gonzalez, B. Central Role of T Helper 17 Cells in Chronic Hypoxia-Induced Pulmonary Hypertension. Am. J. Physiol. Lung Cell Mol. Physiol. 2017, 312(5): L609-L624.
- [35] Luo, X.; Zambaldo, C.; Liu, T.; Zhang, Y.; Xuan, W.; Wang, C.; Reed, S. A.; Yang, PY.; Wang, RE.; Javahishvili, T.; Schultz, PG.; Young, TS. Recombinant thiopeptides containing noncanonical amino acids. Proc. Natl. Acad. Sci. U.S.A., 2016, 113, 3615-20.
- [36] Cao, Y.; Pan, R.; Xuan, W.; Wei, Y.; Liu, K.; Zhou, J.; Wang, W. Photo-triggered Fluorescent Theranostic Prodrug for DNA Alkylating Agent Mechlorethamine Releasing and Spatiotemporal Monitoring. Org. Biomol. Chem., 2015, 13, 6742-6748.
- [37] Zeng, G.; Zhang, R.; Xuan, W.; Wang, W.; Liang, FS. Constructing de novo H2O2 Signaling via Induced Protein Proximity. ACS Chem. Biol., 2015, 10 (6), 1404–1410.