Chen Lei
School
School of Chemical Engineering and Technology
Professional Title
Professor
Contact Information
022-27406364
lchen@tju.edu.cn
135 Yaguan Road, Jinnan District, Tianjin, China
Education Background
- Doctoral degree| Shanghai Institutes for Biological Sciences, CAS| Microbiology| 2008
- Bachelor’s Degree| Shandong University| Biological Science| 2003
Research Interests
- Synthetic Biology; Microbiology; Molecular Biology; Genetic Engineering
Professional Membership
- Dr. Chen has been serving as Review Editor for the journal Frontiers in Microbiology since 2011 Nov, and has peer-reviewed dozens of manuscripts for international journals, including ACS Synthetic Biology, Microbial Cell Factories, Journal of Proteomics.
Positions & Employments
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2011.9-2020.4
School of Chemical Engineering and Technology | Tianjin University, China | Professor -
2008.10-2011.8
Virginia Commonwealth University, USA | Postdoc
Academic Achievements
- Papers
- [1] 23. Sun, T., Xu, L., Wu, L., Song, Z., Chen, L.*, Zhang, W. Identification of a new target slr0946 of the response regulator Sll0649 involving cadmium tolerance in Synechocystis sp. PCC 6803. Frontiers in Microbiology 2017, Accepted. doi: 10.3389/fmicb.2017.01582
- [2] 22. Sun, T., Chen, L.*, Zhang, W. Quantitative proteomics reveals potential crosstalk between a small RNA CoaR and a two-component regulator Slr1037 in Synechocystis sp. PCC6803. Journal of Proteome Research 2017, 16(8):2954-2963.
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- [3] 21. Gao, X., Sun, T., Wu, L., Chen, L.*, Zhang, W. Co-overexpression of response regulator genes slr1037 and sll0039 improves tolerance of Synechocystis sp. PCC 6803 to 1-butanol. Bioresource Technology 2017 May 4, doi: 10.1016/j.biortech.2017.04.112. [Epub ahead of print]
- [4] 20. Chen, L., Ge, X., Wang, X., Patel, J.R., Xu, P. SpxA1 involved in hydrogen peroxide production, stress tolerance and endocarditis virulence in Streptococcus sanguinis. PLoS ONE 2012, 7(6): e40034.
- [5] 19. Chen, L., Zhu, Y., Song, Z., Wang, J., Zhang, W. An orphan response regulator Sll0649 involved in cadmium tolerance and metal homeostasis in photosynthetic Synechocystis sp. PCC 6803. Journal of Proteomics 2014, 103:87-102.
- [6] 18. Chen, L., Wu, L., Zhu, Y., Song, Z., Wang, J., Zhang, W. An orphan two-component response regulator Slr1588 involves salt tolerance by directly regulating synthesis of compatible solutes in photosynthetic Synechocystis sp. PCC 6803. Molecular Biosystems 2014, 10(7):1765-1774.
- [7] 17. Ren, Q., Shi, M., Chen, L.*, Wang, J., Zhang, W. * Integrated proteomic and metabolomic characterization of a novel two-component response regulator Slr1909 involved in acid tolerance in Synechocystis sp. PCC 6803. Journal of Proteomics 2014, 109C:76-89.
- [8] 16. Zhu, Y., Pei, G., Niu, X., Shi, M., Zhang, M., Chen, L.*, Zhang, W*. Metabolomic analysis reveals functional overlapping of three signal transduction proteins in regulating ethanol tolerance in Synechocystis sp. PCC 6803. Molecular Biosystems 2015, 11(3):770-782.
- [9] 15. Niu, X., Zhu, Y., Pei, G., Wu, L., Chen, L.*, Zhang, W.* Elucidating butanol tolerance mediated by a response regulator Sll0039 in Synechocystis sp. PCC 6803 using a metabolomic approach. Applied Microbiology and Biotechnology 2015, 99(4):1845-1857.
- [10] 14. Zhang, Y., Niu, X., Shi, M., Pei, G., Zhang, X., Chen, L.*, Zhang, W.* Identification of a transporter Slr0982 involved in ethanol tolerance in cyanobacterium Synechocystis sp. PCC 6803 . Frontiers in Microbiology 2015, 6:487.
- [11] 13. Yu, X., Niu, X., Zhang, X., Pei, G., Liu, J., Chen, L.*, Zhang, W. *Identification and mechanism analysis of chemical modulators enhancing astaxanthin acc umulation in Haematococcus pluvialis. Algal Research 2015, 11:284-293.
- [12] 12. Gao, X., Sun, T., Pei, G., Chen, L.*, Zhang, W. Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals. Applied Microbiology and Biotechnology 2016, 100(8), 3401-3413.
- [13] 11. Li, T., Zhang, Y., Shi, M., Pei, G. Chen, L.*, Zhang, W.* A putative magnesium transporter Slr1216 involved in sodium tolerance in cyanobacterium Synechocystis sp. PCC 6803. Algal Research, 2016, 17: 202-210.
- [14] 10. Zhang, Y., Pei, G., Chen, L.*, Zhang, W.* Metabolic dynamics of Desulfovibrio vulgaris biofilm grown on steel surface. Biofouling, 2016, 32(7):725-736.
- [15] 9. Fu, Y., Chen, L.*, Zhang, W. Regulatory mechanisms related to biofuel tolerance in producing microbes. Journal of Applied Microbiology 2016, 121(2):320-332.
- [16] 8. Shi, M., Zhang, X., Pei, G., Chen, L.*, Zhang, W. Functional diversity of transcriptional regulators in the cyanobacterium Synechocystis sp. PCC 6803. Frontiers in Microbiology 2017, 8:280.
- [17] 7. Pei, G., Niu, X., Zhou, Y., Chen, L.*, Zhang, W. Crosstalk of two-component signal transduction systems in regulating central carbohydrate and energy metabolism during autotrophic and photomixotrophic growth of Synechocystis sp. PCC 6803. Integrative Biology 2017, 9(5):485-496.
- [18] 6. Li, X., Pei, G., Liu, L., Chen, L.*, Zhang, W. Metabolomic analysis and lipid accumulation in a glucose tolerant Crypthecodinium cohnii strain obtained by adaptive laboratory evolution. Bioresource Technology 2017, 235:87-95.
- [19] 5. Sun, T., Pei, G., Wang, J., Chen, L.*, Zhang, W.* A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance of Synechocystis sp. PCC6803 to 1-butanol possibly via promoter-directed transcriptional silencing. Biotechnology for Biofuels 2017, 10:42.
- [20] 4. Wang, Y., Chen, L.*, Zhang, W*. Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2016, 9:209.
- [21] 3. Chen, L., Wu L., Wang, J., Zhang, W. Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2014, 7:89.
- [22] 2. Song, Z., Chen, L.*, Wang, J., Lu, Y., Jiang, W., Zhang, W.* A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803. Molecular & Cellular Proteomics 2014, 13(12):3519-3532.
- [23] 1. Zhu, H., Ren, X., Wang, J., Song, Z., Shi, M., Qiao, J., Tian, X., Liu, J., Chen, L.*, Zhang, W. Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2013, 6(1):106.
- Books
- [1] (7) Wang, J., Wu, G., Chen, L., Zhang, W. Cross-species transcriptional network analysis reveals conservation and variation in response to metal stress in cyanobacteria. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria. Frans J. de Bruijn, Frans J. de Bruijn, Wiley Online Library. Published Online: 12 AUG 2016. DOI: 10.1002/9781119004813.ch113
- [2] (6) Chen, L., Ren, Q., Wang, J., Zhang, W. Slr1909, a novel two-component response regulator involved in acid tolerance in Synechocystis sp. PCC 6803. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria. Frans J. de Bruijn, Wiley Online Library. Published Online: 12 AUG 2016. DOI: 10.1002/9781119004813.ch91
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- [3] (5) Wang, J., Wu, G., Chen, L., Zhang, W. Integrated analysis of transcriptomic and proteomic datasets reveals information on protein expressivity and factors affecting translational efficiency. Methods in Molecular Biology 2016, 1375:123-136.
- [4] (4) Chen, L., Ge, X., Xu, P. Identifying essential Streptococcus sanguinis genes using genome-wide deletion mutation. Methods in Molecular Biology 2015, 1279:15-23.
- [5] (3) Sun, T., Chen, L., Zhang, W. Proteomic analysis of metabolic responses to biofuels and chemicals in photosynthetic cyanobacteria. Methods in Enzymology 2017;585:355-376.
- [6] (2) Pei, G., Chen, L., Zhang, W. WGCNA application to proteomic and metabolomics data analysis. Methods in Enzymology 2017;585:135-158.
- [7] (1) Chen, Z., Wang, J., Chen, L., Zhang W. RNA-seq based transcriptomic analysis of single cyanobacterial cells. Cyanobacteria: Omics and Manipulation. Edited by Dmitry A. Los. Caister Academic Press, 2017.