Jinhai Huang

Ph D.， DVM，Professor，

Department of Microbiology and Immunology

School of Life Sciences, Tianjin University

No. 92 Weijin Road, Nankai District, Tianjin, China

Cell Phone: (22) 27403902

E-mail: jinhaih@tju.edu.cn

Dr. Huang is interesting in the pathogenic and immunological mechanisms between pathogenic microorganisms (viruses, bacteria and their toxin products) and their host. Especially, he focuses on to discover novel cellular molecules which medicate interaction of pathogens and host, and to understanding the modification regulation roles of them in microbes pathogenesis and immune response. In addition, he aims to understanding the pathogenesis & immunological mechanisms of pathogens and development of integrated monitor and control strategies to improve human and animal health.

Research interests

1) Immunobiology.  Including Mining and functional of novel immune cells and their marker molecules: Based on the big data of omics, to clone and unearth the new molecules of plasma membrane, organelle membrane or cytoplasm, which perform regulation roles as transcription factors, nucleic acid binding proteins, enzymes in the transcription, modification, transport and signal transduction process to understand the mechanism of them in innate immunity and adaptive immune regulation. Design of innate immune regulatory molecular circuits and remodeling of cellular pathways; Design and regulation mechanisms of natural and artificial adjuvants targeted cellular receptors.

(2) Microbial synthetic biology. The design, construction, metabolic regulation and health reshape mechanisms of engineering Probiotics; Large scale transformation and reconstruction technology of resource microbial genome; Research and development of engineered oral mucosal vaccine based on engineering yeast, phage and bacteria; construction of new vaccine design and intelligent manufacturing technology system; Engineering construction, high-throughput screening and application development of animal pathogenic antibody library.

(3) The pathogenic and innate immune mechanism of pathogenic microbes infection. Including the mechanisms of immune escape from viral infection; Rapid rescue of animal virus and development of engineering vaccine; Construction of Predictive vaccinology theory and technique evaluation platform in anti-infection immunity.

Selected papers

2021

l  Yinna Song1#, Yanyu Guo1#, Xiaoyang Li#, Ruiqi Sun, Min Zhu, Jingxuan Shi,Zhen Tan, Lilin Zhang*, Jinhai Huang*， RBM39 alters phosphorylation of c-Jun and binds to viral RNA to promote PRRSV proliferation. Frontiers in Immunology, 2021.12.1-15.

l  Zhe Sun#, Jinhai Huang#, Linjia Su, Jing Li, Fangzheng Qi, Huishan Su, Yanan Chen, Qing Zhang, Qiangzhe Zhang, Zongjin Li, Sihe Zhang, Arf6-mediated macropinocytosis-enhanced suicide gene therapy of C16TAB-condensed Tat/ pDNA nanoparticles in ovarian cancer. Nanoscale, 2021.1-14. 

l  Hui Zhai#, Jingxuan Shi#, Ruiqi Sun, Zheng Tan, Umm E Swaiba, Wanqing Li, Lilin Zhang, Lei Zhang，Yanyu Guo*, Jinhai Huang*，The Superposition Anti-viral Activity of Porcine Tri-subtype Interferon Expressed by Saccharomyces cerevisiae. Veterinary microbiology. 2021

l  Min Zhu#, Xiaoyang Li#, Yinna Song, Peidian Shi, Aiping Cao, Lilin Zhang, yanyu Guo*, Jinhai Huang*, The C/EBPβ-Dependent Induction of TFDP2 Facilitates Porcine Reproductive and Respiratory Syndrome Virus Proliferation. Virologica Sinica, 2021.6,1-11. 

l  Jingxuan Shi #, Heng Chi#, Aiping Cao, Min Zhu, Linlin Zhang, Fuzhou Xu*, Jinhai Huang*，Development of IMBs-qPCR Detection Method for Yersinia enterocolitica Based on the foxA Gene. Archives of Microbiology. 2021, 1-10.

l  Xiaoyang Li#, Yanyu Guo#, Yinna Song#, Ruiqi Sun, Min Zhu, Zheng Tan, Umm E Swaiba, Lilin Zhang, Jinhai Huang*,The Glycosyltransferase ST3GAL2 Modulate the Proliferation and Inflammation Response in PRRSV Infection，Archives of Virology, 2021,1-10.

l  Chen Chen#, Deping Hua#, Jingxuan Shi, Zheng Tan, Min Zhu, Kun Tan, Lilin Zhang, Jinhai Huang*，Porcine immunoglobulin Fc fused P30/P54 protein of African swine fever virus displaying on surface of S. cerevisiae elicit strong antibody production in swine. Virologica Sinica, 2021;36 (2):207-219.

2020

l  Aiping Cao, Heng Chi, Jingxuan Shi ,Ruiqi Sun, Kang Du, Yinna Song,Min Zhu, Lilin Zhang, and Jinhai Huang* Visual detection of Clostridium perfringens alpha toxin by combining nanometer microspheres with smart phones. Microorganisms 2020,8 (12),1865. 

l  Jiaqi Wu#, HengChi#, Yanyu Guo, Aiping Cao, Jingxuan Shi, Min Zhu, Lilin Zhang, DepingHua*, Jinhai Huang*, Antiviral Protein Viperin Modulate N protein to Inhibit Porcine Epidemic Diarrhea Virus proliferation. Archives of Virology, 2020, 165:2279–2289

l  Peidian Shi, Yanyu Guo, Yanxin Su, Min Zhu, Yali Fu, Heng Chi, Jiaqi Wu, Jinhai Huang*，SUMOylation of DDX39A alters binding & export of antiviral transcripts to control innate immunity. Journal of Immunology, 2020, 205（1）：168-180. 

l  Yanyu Guo, Ruiqiao Li, Zheng Tan, Jingxuan Shi, Yali Fu, Yinna Song, Min Zhu, Lei Zhang, Jinhai Huang*，E3 ubiquitin ligase ASB8 negatively regulates interferon via regulating TBK1/IKKi homeostasis. Molecular Immunology. 2020, Vol 121, 195-203. 

l  Yali Fu, Dong Lu,· Yanxin Su,, Heng Chi, Jiashun Wang, Jinhai Huang*，Vif Protein of Caprine Arthritis Encephalitis Virus Inhibits Interferon Production, Archives of Virology. 2020, 1-11.

2019

l   Ruiqiao Li#, Chen Chen#, Jing He, Lilin Zhang, Lei Zhang, Yanyu Guo, Jinhai Huang*, E3 Ligase ASB8 Promotes PRRSV Proliferation and Function via Stabilizing of Nsp 1α and Degrading of IKK. Virology, 2019，532：55–68.

lXiao Xiao,Wentao Zhang, Deping Hua, Lilin Zhang, Wei Meng, Jinhai Huang*, Lei Zhang*, Cold-inducible RNA-binding protein (CIRBP) promotes porcine reproductive and respiratory syndrome virus (PRRSV)-induced inflammatory response. International Immunopharmacology. 2020, 86:106728.

l  Chengxue Zhao#, Chen Chen#, Ying Li, Shuren Dong, Kun Tan,,Yuhui Tian,,Lilin Zhang, Jinhai Huang*, Lei Zhang*, Genomic characterization of a novel recombinant porcine astrovirus isolated in northeastern China. Archives of Virology, 2019, 164(3): 1469-1473.

l  Peidian shi#; Yanxin Su#; Yi Li#; Lilin Zhang; Dong Lu; Ruiqiao Li; Lei Zhang; Jinhai Huang*，The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production. Developmental and Comparative Immunology, 2019, 90: 186~196.

l  Peidian Shi1#, Yanxin Su1#, Ruiqiao Li1, Zhixuan Liang, Shuren Dong, Jinhai Huang*，PEDV nsp16 negatively regulates innate immunity to promote viral proliferation. Virus Research, 2019, 265，57–66.

2018

l  Yanxin Su(#); Peidian Shi(#); Lilin Zhang; Dong Lu; Chengxue Zhao; Ruiqiao Li; Lei Zhang*; Jinhai Huang*,The Superimposed Deubiquitination Effect of OTULIN and PRRSV Nsp11 Promoted the Multiplication of PRRSV. Journal of Virology, 2018.2.12, 92(9): 1-17.

l  Peidian Shi(#); Yanxin Su(#); Ruiqiao Li; Lei Zhang; Chen Chen; Lilin Zhang; Kay Faaberg*; Jinhai Huang*, Dual Regulation of Host TRAIP Post-translation and Nuclear/Plasma Distribution by Porcine Reproductive and Respiratory Syndrome Virus Non-structural Protein 1a Promotes Viral Proliferation. Frontiers in Immunology, 2018.12.6, 9(3023): 1~18.

l  Peidian Shi(#); Lilin Zhang(#); Jiashun Wang(#); Dong Lu; Yi Li; Jie Ren; Menglu Shen; Lei Zhang; Jinhai Huang*, Porcine FceRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction. Virologica Sinica (2018) 33:249–260.

l  Chen Pei(#); Zhang Lei(#); Chang Na; Shi Peidian; Gao Tian; Zhang Lilin*, Huang Jinhai*, Preparation of virus-like particles for porcine circovirus type 2 by YeastFab Assembly. Virus Genes, 2018.4, 54(2): 246~255.

l  Lei Zhang#; Jie Ren#; Peidian Shi; Dong Lu; Chengxue Zhao; Yanxin Su; Lilin Zhang; Jinhai Huang*, The Immunological Regulation Roles of Porcine b-1, 4 Galactosyltransferase V (B4GALT5) in PRRSV Infection. Frontiers in Cellular and Infection Microbiology, 2018.2.9, 8(48): 1~13.

l  Ruiqiao Li, Xuefeng Qi,Xueying Han,Caihong Liu, Jing Wang, Ruichun Wang, Jingyu Wang*, Jinhai Huang*,Deterioration of eggshell quality is related to calbindin in laying hens infected with velogenic genotype VIId Newcastle disease virus. Theriogenology.2017,91(3):62-68. IF: 2.288,  Q1, 26/142.

Before 2017 

l  Yi Li, Hui Deng, Pei-Dian Shi, Dan-Dan Xu, Li-Lin Zhang, Lei Zhang*, Jinhai Huang* , Toxic effects of seu are different from those of other staphylococcal enterotoxins. American Journal of Translational Research. 2017, 9(4):1618-1629.

l  Jiashun Wang^#, Yi Li^1#, Hui Deng, Jia Chen, Ying Li, Zhixuan Liang, Deping Hua, Jinhai Huang*. Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene. Brazilian Journal of Microbiology, 2017.10:3241-9.

l  Menglu Shen, Yi Li, Linlin Zhang, Songbao Dai, Jiashun Wang, Yongqing Li, Lei Zhang*, Jinhai Huang*，Staphylococcal enterotoxin Profile of China Isolates and the Superantigenicity of Some Novel Enterotoxins，Archives of Microbiology.2017, 199: 1-14.

l  Ruiqiao Li,† Lilin Zhang,† Peidian Shi, Hui Deng, Yi Li, Jie Ren, Xubin Fu, Lei Zhang*, Jinhai Huang，Immunological Effects of Different Types of Synthetic CpG Oligodeoxynucleotides on Porcine Cells. RSC advances .2017, 7, 43289-99.

l  Hongzhi Kang, Hui Deng, Menglu Shen, Xianzhi He, Yi Li,Yihe xia, Hongjun Wang, Zhixuan Liang, Jinhai Huang* Superantigenicity analysis of Staphylococcal Enterotoxins SElK and SElQ in a mouse model. RSC advances .2015.5, 29684–29692.

l  Qiangzhe Zhang, Caihong Zhang, Mengxu Gao, Xianzhi He, Yinghua Diao, Sagar M Goyal, Sunil K. Mor, Jinhai Huang*, Evolutionary, epidemiological, demographical, and geographical dissection of porcine bocavirus in China and America. Virus Research. 2015,195 (1): 13–24.

l  Shiwen Zhuang, Junshu Fu, Chris Powell, Jinhai Huang*, Yihe Xia, Ruixiang Yan, Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1. SpringerPlus, 2015, 4:467-476.

l  Xianzhi He, Lei Zhang, Pengchong Liu, Li Liu, Hu Deng, Jinhai Huang*, Construction and Characterization of VL-VH Tail-parallel Genetically Engineered Antibodies against Staphylococcal Enterotoxins. Immunologic Research.2015,61(3): 281-293.

l  Jianming Wu, Rui Lin, Jinhai Huang, Weihua Guan, William S. Oetting, P. Sriramarao, Malcolm N. Blumenthal, Functional Fcgamma Receptor Polymorphisms Are Associated with Human Allergy. PLOS ONE,2014,Vol 9(2): e89196.

l  Jia Chen, Xianzhi He, Jinhai Huang*, Diet effects in gut microbiome and obesity, Journal of Food Science. 2014，Vol 79（4）: 442–451.

l  Mengmeng Liu, Yi Li,Yihe Xia, Jinhai Huang*,Lili Guo, Detection and genetic analysis of Porcine Bocavirus in different swine herds in North Central China. The world science journal.2014，947084.

l  Jinhai Huang, Sunil K. Mor, Jonathan Erber, Elyce Voss, Sagar M. Goyal, Detection and characterization of porcine bocavirus in United States. Archives of Virology, 2014,159 (7):1797-1801.

l  Jinhai Huang, Yi Li, Mengmeng Liu, Yihe Xia, Zhirong Li, A Novel Subgenotype of Torque teno Virus 1 (TTSuV1) in Slaughter Pigs in China. Food and environmental virology. 2013,226-230.

l  Xu Cong,Junfeng Jiang,Tiegen Liu, Kun Liu,Pengchong Liu,Jinhai Huang,Qiang Ji,Yimo Zhang, Characterization of immobileization of staphylococcal enterotoxin an antibody on microtube. Sensor Letters, 2012, Vol 10:1523-1527.

l  Yi Li, Fengjuan Zhou, Xia Li, Jianhua Wang, Xiangping Zhao,Jinhai Huang*， Development of TaqMan-based qPCR method for detection of caprine arthritis-encephalitis virus (CAEV) infection. Archives of Virology, 2013，158:2135-2141.

l  Jin-Hai Huang,Yue-Hui Sun,Yebing Liu,Huazhi Xiao,Shiwen Zhuang，Development of a loop-mediated isothermal amplification method for rapid detection of Caprine arthritis encephalitis virus proviral DNA. Archives of Virology, 2012. 157（8）:1463-1469.

l  Jianming Wu, Maureen Richards; Jinhai Huang; Lena Al-Harthi; Xiulong Xu; Rui Lin; Fenglong Xie; Andrew W. Gibson; Jeffrey C. Edberg; Robert P. Kimberly. Human, FasL Gene is a Target of β-catenin/T-cell Factor Pathway and Complex FasL Haplotypes Alter Promoter Functions". PLoS ONE, 2011，Vol 6（10）：e26143.

l  Han Xueqing，Lin Xiangmei，Liu Bohua，Hou Yihong, Huang Jinhai，et al，Simultaneously subtyping of all influenza A viruses using DNA microarrays，Journal of Virological Methods，2008，152(1-2)：117–121.

Authorized Patents

• China/ZL201110049950.X: The real-time PCR method to differentiating classic and high virulent strains of porcine reproductive and respiratory syndrome virus, 2015.12.9.

• China/ZL200710064255.4: Gene chips for detection and genotyping of avian influenza viruses.2010.13

• China/ ZL2006100894635: aGST-ELISA method to detect avian influenza virus antibodies.  2009.9

• China/ ZL200620027159.3: A new lamb incubator, 2006.8.29

• China/ ZL200620027282.5: A sponge pessary implant device used for goats, 2006.9

• China/ ZL200620027751.3: A device with light for dairy vagina detection, 2006.10.25

• China/ ZL200620027281.0: A calf raising bed, 2006.10.25

  
  

Selected Published Books 

• Huang Jinhai (chief editor), Principles of Molecular Biology & Practical Application. China Atomic Press.2015.12

• Changxin Wu, et.al., 10000 Selected Problems in Sciences—Agriculture Ssciences. China Science Press.2011.

• Wang Jiaxin, Qin Aijian, Huang Jinhai, et. al., Immunology, China Agriculture Press.2009.

• Diao Enjie, Xu Baocheng, Huang Jinhai. et. al., Food Safety and Quality Management. Chemistry Industry Press.2008.

• Li Zhirong, Ding Shuangyang, Huang Jinhai. et. al., Detection Techniques of Veterinary Drug Residues. Tianjin Science and Technology Press.2008.

• Shi Zhicheng, Wang Jianhua, Huang Jinhai. et. al., Animal Toxiology, China Agriculture Press.2002.

Research Projects

• “Biosythesis of Key proteins used in veterinary medicine”(TSBICIP-KJGG-014)，supported by Synthetic biotechnology innovation capability improvement project.

• R&D of multi-valent avian influenza vaccine based on Saccharomyces cerevisiae chassis”(20YFZCSN00340)., supported by underprop project of Tianjin Science and Technology committee in Tianjin,China.
  

• “Molecular mechanism of multisystem inflammation syndrome caused by staphylococcal enterotoxins”，supported by National Science Fund in China (31272540）

• “Study on the virus-like particle vaccine of porcine circovirus type 2 using self-designed S. cerevisiae system”, supported by underprop project of Tianjin Science and Technology committee in Tianjin (16YFZCNC00640)

• “ Techniques of cell suspension culture in animal vaccine preparation”, Tianjin science and technology support key project plan(13ZCZDNC08800)

• “ Immobiltzation of Recombinant Chitinase with Magnetic Carrier and Analysis on Structure and Effect”(31101362)，supported by National Science Fund in China

• “Development of genotyping method and high-output detection kits of staphylococcal enterotoxins in food”(08JCZDJC22600) , supported by a grant from the application basement & advanced technology project of Tianjin Science and Technology committee in Tianjin ,China.

• “R&D of a recombinant of porcine antimicrobial Peptide”(07ZCKFNC00600)., supported by underprop project of Tianjin Science and Technology committee in Tianjin,China.

•  “Study on pathogen variation and control measure of procine productive and respiratory syndrome in Tianjin region ” (08JCZDJC22900), supported by a grant from the application basement & advanced technology project of Tianjin Science and Technology committee in Tianjin ,China.

• “Molecular mechanisms of immune response of goats infected with caprine arthritis encephalitis virus”，supported by national Science fund (30771613) in China.

• “Research on the protein-chip for diagnosis of some important bovine infectious diseases”, 06YFGZSH02100, supported by Tianjin Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China.

• “Study on of the molecular diagnosis kits for diagnosis of caprine arthritis encephalitis virus ”supported by General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China.

• “Construction of recombinant plasmid of chicken Marek's disease virus and preparation of major protective antigen protein”, 2013GKF-0482, Beijing academy of agriculture science.

• “Rapid detection of Foot and mouth disease virus, avian influenza virus by Helicase-dependent Isothermal DNA Amplification(HAD) techniques”, 2009IK021,Beijing Administration of Quality Supervision, inspection and Quarantine of the People's Republic of China.

• “Diagnosis & control key techniques of animal epidemic diseases”, a collaboration plan of foreign expert, supported by Tianjin government.

  
  

Professional Membership

Member of World Poultry Congress, Chinese Microorganism Association, and Chinese Association of Poultry Diseases.

Technique consultant for Tianjin Animal Disease Risk Assessment, and Quality and Safety Testing Center for agricultural products of Tianjin.

Reviewer for:

• PLOS pathogens

• Journal of Agriculture and Food Chemistry

• Veterinary microbiology

• Virologica Sinica

• Archives of virology

• Journal of virological methods

• BMC Complementary and Alternative Medicine

• Journal of Veterinary Science & Technology.

• Small Ruminant Research

• Journal of the Royal Society Interface

• Journal of 3Biotech

• Journal of food safety

• Chinese journal of veterinary medicine

• Online Chinese journal of scientific papers.

  
  

Honors and Awards

• 2012: "Key technologies in modern pig breeding integrated demonstration and promotion", the third prize of Tianjin progress prize in scientific and collective technology by Tianjin government.

• 2011: “Research & development of a recombinant antibacterial peptide”, the first prize of Dongli District progress prize in scientific and collective technology by Dongli District government of Tianjin.

• 2012: "Noval estrus suppository used for cattle and goats ", the second Invention prize of Tianjin Binhai district of Tianjin.

• 2011: “Development of diagnosis kits in genotype identification and high-throughput detection in staphylococcal enterotoxin”, Tianjin scientific and technological achievements in 2011.

• 2016: “Techniques of cell suspension culture in animal vaccine preparation” , Tianjin scientific and technological achievements in 2016.

• 2001: “The pathogen isolation and vaccine development of main poultry infectious diseases in Tianjin region”. the second prize of Tianjin progress prize in scientific and collective technology by Tianjin government.

• 2000："The comprehensive prevention and control technology research——the heterologous cell inactivated vaccine and diagnostic kit for chicken infectious bursal disease"，the first prize of Tianjin progress prize in scientific and collective technology by Shaanxi province government.

• 2017: "The good supervisor in my mind "(Tenth session), Tianjin University.

• 2015: The best teacher in 2015 of School of Life Sciences, Tianjin University.

• 2014: Individual advanced teaching and education award of Tianjin University.